Part:BBa_K3790050:Design

T7 phage gene product 5.7, an E.coli transcription inhibitor (RFC compatible)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The following primers were used for constructing this part.

gp5.7_coding1	5-GTCCAACTATGTACGGAACAATGCGAGCCTCGTAGCGGAGGCCGCTTCCCGTGGTCAC
gp5.7_coding2	5-CTGCAAGCAATCAAAAGTTGCCCTAAGACTTTCCAGTCCAACTATGTACGGAACAATGC
gp5.7_coding3	5-ATGTCTGACTACCTGAAAGTGCTGCAAGCAATCAAAAGTTGCC
gp5.7_template1	5-CCCAAGCGCCACCGTTACGTCCgCTAGTAGTCAGGCACGAGATGTGACCACGGGAAGCG
gp5.7_template2	5-CATTCGTTTCAGAAAGCGAGTACCGGAAGCAGTGATTTCCCAAGCGCCACCG
gp5.7_template3	5-TTAGACACATCCTCCCATTCGTTTCAGAAAGCGAGTACC
doubleter_f	5-CGATATAAGTTGTAATTCTCaaataataaaaaagccggattaataatctggc
doubleter_r	5-GAAGGCTCTCAAGGGCATtcacactggctcaccttcg
gp5.7_f		5-gaATGCCCTTGAGAGCCTTCTTAGACACATCCTCCCATTCGTTTC
gp5.7_r		5-gagttaaggaggtaaaaaaaATGTCTGACTACCTGAAAGTGCTG